High-performance liquid chromatographic methods for quantitative assessment of degradation products and extractives in pretreated lignocellulose.

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dc.contributor.advisor Chambliss, C. Kevin. Chen, Shou-Feng.
dc.contributor.other Baylor University. Dept. of Chemistry and Biochemistry. en 2007
dc.identifier.citation Chen, S-F.; Mowery, R. A.; Castleberry, V. A.; van Walsum, G. P.; Chambliss, C. "High-performance liquid chromatography method for simultaneous determination of aliphatic acid, aromatic acid and neutral degradation products in biomass pretreatment hydrolysates." Journal of Chromatography A, vol. 1104 no. 1-2 (Feb. 3, 2006), 54-61. en
dc.identifier.citation Chen, S-F.; Mowery, R. A.; Scarlata, C. J.; Chambliss, C. K. "Compositional analysis of water-soluble materials in corn stover." Journal of agricultural and food chemistry, vol. 55 no. 15 (July 25, 2007). en
dc.description.abstract Long-term economic, national security, and environmental concerns have motivated research into renewable fuels from lignocellulosic biomass. Among energy alternatives, biomass-derived ethanol represents one of the more promising commodities for long-term sustainability of transportation fuels. Herbaceous agricultural residues, such as corn stover, represent a major source of lignocellulosic material with considerable potential for use in biomass-to-ethanol schemes. Currently, the technology for conversion of biomass to ethanol involves dilute acid pretreatment of lignocellulosic materials, followed by enzymatic hydrolysis of cellulose and fermentation of monomeric sugars to produce ethanol. However, a variety of degradation products are produced upon dilute acid pretreatment of lignocellulosic biomass, which exert an inhibitory effect on downstream fermentation processes and reduce bioethanol conversion. Thus there is increased the demand for reliable analytical methods to advance a more understanding of lignocellulose pretreatment. Several liquid chromatographic methods are developed for a systematic analysis of various degradation products. High-performance liquid chromatography is the most widely used analytical separation technique, because of its reproducibility, sensitivity, and suitability for separating nonvolatile species, which makes the method ready for accurate quantitative determinations. A reversed-phase HPLC method with UV detection is developed for simultaneous separation and quantitation of organic acids and neutral degradation products present in the corn stover hydrolysate. On the other hand, inorganic ions and some organic anions which present in water extractive from corn stover are separated and quantitated by the developed ion chromatographic method with conductivity mode. Sugars and alditols are determined using high-performance anion chromatography with pulsed amperometric detection. en
dc.publisher Amsterdam : Elsevier B. V. en
dc.publisher Washington, D.C. : American Chemical Society. en
dc.rights Baylor University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact for inquiries about permission. en
dc.subject Lignocellulose -- Biodegradation. en
dc.subject Lignocellulose -- Biotechnology. en
dc.subject High performance liquid chromatography. en
dc.subject Biomass energy. en
dc.title High-performance liquid chromatographic methods for quantitative assessment of degradation products and extractives in pretreated lignocellulose. en
dc.type Thesis en Ph.D. en
dc.rights.accessrights Worldwide access en
dc.contributor.department Chemistry and Biochemistry. en

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