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Determination of pharmaceuticals in fish and wastewater using isotope dilution high performance liquid chromatography-tandem mass spectrometry.

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dc.contributor.advisor Chambliss, C. Kevin.
dc.contributor.author Hurtado, Pilar Perez.
dc.contributor.other Baylor University. Dept. of Chemistry and Biochemistry. en
dc.date.copyright 2009-05
dc.identifier.uri http://hdl.handle.net/2104/5345
dc.description.abstract A method has been developed for the trace analysis of 15 pharmaceuticals and employs liquid chromatography/tandem mass spectrometry (LC-ESI-MS/MS). Unlike many previous methods, which suffer from matrix suppression and recovery loss, the present method utilizes isotope dilution for each compound to correct for these phenomena. Matrix spike recoveries for all compounds were between 87 and 113% for wastewater effluent, 98 and 144% for fish fillet tissue, and 81% and 105% for fish liver tissue. Method Detection limits (MDLs) in wastewater, fish fillet, and liver tissue were ≤21, ≤13, and ≤41 ng/g, respectively for most analytes. Additionally, this method was directly compared with matrix matched calibration to evaluate potential to compensate for matrix effects. Matrix matched was not suitable to account for matrix effects in samples with highly loaded matrix such as fish liver, though this method offered greater sensitive compared to isotope dilution in the detection of some compounds such as norfluoxetine, sertraline, and fluoxetine. en
dc.rights Baylor University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact librarywebmaster@baylor.edu for inquiries about permission. en
dc.subject Drugs -- Environmental aspects. en
dc.subject Drugs -- Toxicology. en
dc.subject Water -- Pollution. en
dc.subject Fishes -- Effect of water pollution on. en
dc.subject Fishes -- Physiology. en
dc.subject High performance liquid chromatography. en
dc.subject Mass spectrometry. en
dc.subject Isotope dilution analysis. en
dc.title Determination of pharmaceuticals in fish and wastewater using isotope dilution high performance liquid chromatography-tandem mass spectrometry. en
dc.type Thesis en
dc.description.degree M.S. en
dc.rights.accessrights Baylor University access only en
dc.contributor.department Chemistry and Biochemistry. en


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