Inhibition of β-lactamase I from Bacillus cereus by ssDNA.

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dc.contributor.advisor Kim, Sung-Kun, 1968- Foster, Taylor Ann. 2011-08
dc.description.abstract Inhibitors of β-lactamases are important to the treatment of infectious diseases when used in conjunction with a β-lactam antibiotic. Current inhibitors of β-lactamase such as clavulanic acid, sulbactam, and tazobactam perform efficiently overall but due to developing bacterial resistances to these inhibitors, new inhibitors need to be discovered. SELEX procedures were used to isolate ssDNA aptamers capable of binding to the enzyme active site and consequently inhibit the action of β-lactamase I from Bacillus cereus 569/H/9. A 22 base ssDNA aptamer was discovered to have an inhibition pattern consistent with reversible competitive inhibition. These results prompted further study of a hairpin loop of 10 bases and a linear 11 base ssDNA aptamer which were truncated forms of the original 22 base aptamer. The 11 base aptamer failed to show any inhibition against β-lactamase I, whereas the 10 base aptamer showed competitive reversible inhibition. en_US
dc.publisher en
dc.rights Baylor University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact for inquiries about permission. en_US
dc.subject Beta-lactamase inhibitor. en_US
dc.subject SELEX. en_US
dc.subject ssDNA. en_US
dc.title Inhibition of β-lactamase I from Bacillus cereus by ssDNA. en_US
dc.type Thesis en_US M.S. en_US
dc.rights.accessrights Worldwide access. en_US
dc.rights.accessrights Access changed 1/14/14.
dc.contributor.department Biomedical Studies. en_US
dc.contributor.schools Baylor University. Institute of Biomedical Studies. en_US

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