CTR2 and the nonsense-mediated mRNA decay pathway in Saccharomyces cerevisiae.

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dc.contributor.advisor Kebaara, Bessie Wanja. Wang, Xuya. 2012-08
dc.description.abstract The nonsense-mediated mRNA decay (NMD) pathway recognizes and degrades mRNA with premature termination codon and some natural mRNA as well. CTR2 is a natural mRNA degraded by NMD in Saccharomyces cerevisiae. The goals of this research were to identify the sequence features that target the CTR2 mRNA for NMD and the physiological consequences resulting from this degradation. These goals were addressed by making fusion constructs and determining total cellular, cytoplasmic and vacuolar copper levels in wild-type and nmd mutant yeast cells. Features contribute to the NMD-mediated degradation of CTR2 were identified. When cultured in medium with excess copper, nmd mutants accumulated significantly higher vacuolar copper levels than wild-type yeast cells, however nmd mutants accumulated significantly less cytoplasmic copper levels than that in wild-type yeast cells. These results are consistent with the inference that nmd mutants tolerate excess copper as a result of ctr2p transporting excessive copper from cytoplasm into vacuole. en_US
dc.publisher en
dc.rights Baylor University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact for inquiries about permission. en_US
dc.subject Nonsense-mediated mRNA decay. en_US
dc.subject CTR2. en_US
dc.title CTR2 and the nonsense-mediated mRNA decay pathway in Saccharomyces cerevisiae. en_US
dc.type Thesis en_US M.S. en_US
dc.rights.accessrights Worldwide access. en_US
dc.rights.accessrights Access changed 1/14/14.
dc.contributor.department Biology. en_US
dc.contributor.schools Baylor University. Dept. of Biology. en_US

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